Occurrence and operation of the glycollate--glyoxylate shuttle in mitochondria of Euglena gracilis Z.
نویسندگان
چکیده
Both glyoxylate reductase (NADP+) and glycollate dehydrogenase were located exclusively in mitochondria in Euglena gracilis and constitute the glycollate--glyoxylate shuttle, whose existence in higher plants was thought doubtful, owing to different subcellular locations of the two enzymes. Disrupted Euglena mitochondria showed a glycollate-dependent NADPH oxidation, indicating actual operation of the shuttle in this protozoon.
منابع مشابه
Purification and some properties of glyoxylate reductase (NADP+) and its functional location in mitochondria in Euglena gracilis z.
Euglena mitochondria contain both glyoxylate reductase (NADP+) and glycollate dehydrogenase to constitute the glycollate-glyoxylate cycle [Yokota & Kitaoka (1979) Biochem. J. 184, 189-192]. Euglena glyoxylate reductase (NADP+) was purified and its submitochondrial location was determined in order to elucidate the cycle. The purified glyoxylate reductase was homogeneous on polyacrylamide-gel ele...
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7. Cook, J. R. 1968. The cultivation and growth of Euglena, in Buetow, D. E., ed., The Biology of Euglena, Academic Press, New York 1, 243-314. 8. Cooper, T. G. & Beevers, H. 1969. P-oxidation in glyoxysomes from castor bean endosperm. J . Biol. Chem. 244, 3514-20. 9. Cramer, M. & Myers, J. 1952. Growth and photosynthetic characteristics of Euglena gracilis. Arch. Mikrobiol. 17, 384-402. 10. Da...
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عنوان ژورنال:
- The Biochemical journal
دوره 184 1 شماره
صفحات -
تاریخ انتشار 1979